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Table 2 Cross-reactivity of horse sera between ELISA and VNT

From: Tick-borne encephalitis virus (TBEV) – findings on cross reactivity and longevity of TBEV antibodies in animal sera

 

TBEV-ELISA

TBEV-VNT

WNV-ELISA

WNV-VNT

Sample

U/l 1

Result

ND 50

S/N%

Result

ND 50

Serum 1

-2,995

Negative

n.d.

92,25%

Negative

< 1:10

Serum 2

12,500

Borderline

>1:640/1:960

11,99%

Positive

< 1:10

Serum 3

-2,938

Negative

n.d.

88,04%

Negative

< 1:10

Serum 4

-3,802

Negative

n.d.

94,11%

Negative

< 1:10

Serum 5

3,859

Negative

n.d.

41,48%

Borderline

< 1:10

Serum 6

17,627

Positive

1:80

7,27%

Positive

< 1:10

Serum 7

-2,650

Negative

n.d.

77,31%

Negative

< 1:10

Serum 8

-3,571

Negative

n.d.

86,93%

Negative

< 1:10

Serum 9

-3,168

Negative

n.d.

90,48%

Negative

< 1:10

Serum 10

3,111

Negative

n.d.

3,16%

Negative

< 1:10

Serum 11

-0,806

Negative

n.d.

93,43%

Negative

< 1:10

Serum 12

-3,975

Negative

n.d.

93,54%

Negative

< 1:10

Serum 13

-2,995

Negative

n.d.

92,59%

Negative

< 1:10

Serum 14

-3,744

Negative

n.d.

89,52%

Negative

< 1:10

Serum 15

-2,995

Negative

n.d.

88,38%

Negative

< 1:10

Serum 16

4,896

Negative

n.d.

86,58%

Negative

< 1:10

Serum 17

-3,168

Negative

n.d.

85,80%

Negative

< 1:10

Serum 18

-2,765

Negative

n.d.

91,29%

Negative

< 1:10

Serum 19

17,915

Positive

1:80

15,04%

Positive

< 1:10

Serum 20

20,392

Positive

1:240

51,14%

Negative

< 1:10

Serum 21

12,212

Borderline

1:160

13,51%

Positive

< 1:10

Serum 22

17,569

Positive

1:320

9,48%

Positive

< 1:10

Serum 23

-3,053

Negative

n.d.

84,21%

Negative

< 1:10

Serum 24

-3,399

Negative

n.d.

83,57%

Negative

< 1:10

Serum 25

16,993

Positive

1:80

7,06%

Positive

< 1:10

Serum 26

-4,818

Negative

n.d.

89,77%

Negative

< 1:10

Serum 27

-5,867

Negative

n.d.

83,66%

Negative

< 1:10

Serum 28

-3,959

Negative

n.d.

84,43%

Negative

< 1:10

Serum 29

-5,295

Negative

n.d.

91,08%

Negative

< 1:10

Serum 30

10,351

Borderline

<1:5

86,85%

Negative

< 1:10

Serum 31

-5,200

Negative

n.d.

86,23%

Negative

< 1:10

Serum 32

-4,532

Negative

n.d.

86,37%

Negative

< 1:10

Serum 33

31,245

Positive

>1:40/1:720

27,45%

Positive

< 1:10

Serum 34

14,168

Positive

>1:40/1:480

76,10%

Negative

< 1:10

Serum 35

21,991

Positive

1:40

40,69%

Borderline

< 1:10

Serum 36

35,443

Positive

>1:40/>1:960

21,43%

Positive

< 1:10

Serum 37

-5,581

Negative

n.d.

86,84%

Negative

< 1:10

Serum 38

-4,913

Negative

<1:5

88,36%

Negative

< 1:10

Serum 39

35,538

Positive

>1:40/1:480

6,68%

Positive

< 1:10

Serum 40

30,291

Positive

>1:40/1:960

17,62%

Positive

< 1:10

Serum 41

-5,200

Negative

<1:5

85,33%

Negative

< 1:10

Serum 42

-3,864

Negative

n.d.

86,76%

Negative

< 1:10

Serum 43

-5,486

Negative

n.d.

87,18%

Negative

< 1:10

Serum 44

-3,959

Negative

n.d.

87,91%

Negative

< 1:10

Serum 45

-5,200

Negative

n.d.

86,37%

Negative

< 1:10

Serum 46

13,691

Positive

>1:40/>1:960

3,07%

Positive

< 1:10

  1. In bold = TBEV-positive horses would result in false positive outcomes in the commercial WNV-ELISA, but clearly negative in the WNV-specific VNT.
  2. n.d. = not done.