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Table 2 Cross-reactivity of horse sera between ELISA and VNT

From: Tick-borne encephalitis virus (TBEV) – findings on cross reactivity and longevity of TBEV antibodies in animal sera

  TBEV-ELISA TBEV-VNT WNV-ELISA WNV-VNT
Sample U/l 1 Result ND 50 S/N% Result ND 50
Serum 1 -2,995 Negative n.d. 92,25% Negative < 1:10
Serum 2 12,500 Borderline >1:640/1:960 11,99% Positive < 1:10
Serum 3 -2,938 Negative n.d. 88,04% Negative < 1:10
Serum 4 -3,802 Negative n.d. 94,11% Negative < 1:10
Serum 5 3,859 Negative n.d. 41,48% Borderline < 1:10
Serum 6 17,627 Positive 1:80 7,27% Positive < 1:10
Serum 7 -2,650 Negative n.d. 77,31% Negative < 1:10
Serum 8 -3,571 Negative n.d. 86,93% Negative < 1:10
Serum 9 -3,168 Negative n.d. 90,48% Negative < 1:10
Serum 10 3,111 Negative n.d. 3,16% Negative < 1:10
Serum 11 -0,806 Negative n.d. 93,43% Negative < 1:10
Serum 12 -3,975 Negative n.d. 93,54% Negative < 1:10
Serum 13 -2,995 Negative n.d. 92,59% Negative < 1:10
Serum 14 -3,744 Negative n.d. 89,52% Negative < 1:10
Serum 15 -2,995 Negative n.d. 88,38% Negative < 1:10
Serum 16 4,896 Negative n.d. 86,58% Negative < 1:10
Serum 17 -3,168 Negative n.d. 85,80% Negative < 1:10
Serum 18 -2,765 Negative n.d. 91,29% Negative < 1:10
Serum 19 17,915 Positive 1:80 15,04% Positive < 1:10
Serum 20 20,392 Positive 1:240 51,14% Negative < 1:10
Serum 21 12,212 Borderline 1:160 13,51% Positive < 1:10
Serum 22 17,569 Positive 1:320 9,48% Positive < 1:10
Serum 23 -3,053 Negative n.d. 84,21% Negative < 1:10
Serum 24 -3,399 Negative n.d. 83,57% Negative < 1:10
Serum 25 16,993 Positive 1:80 7,06% Positive < 1:10
Serum 26 -4,818 Negative n.d. 89,77% Negative < 1:10
Serum 27 -5,867 Negative n.d. 83,66% Negative < 1:10
Serum 28 -3,959 Negative n.d. 84,43% Negative < 1:10
Serum 29 -5,295 Negative n.d. 91,08% Negative < 1:10
Serum 30 10,351 Borderline <1:5 86,85% Negative < 1:10
Serum 31 -5,200 Negative n.d. 86,23% Negative < 1:10
Serum 32 -4,532 Negative n.d. 86,37% Negative < 1:10
Serum 33 31,245 Positive >1:40/1:720 27,45% Positive < 1:10
Serum 34 14,168 Positive >1:40/1:480 76,10% Negative < 1:10
Serum 35 21,991 Positive 1:40 40,69% Borderline < 1:10
Serum 36 35,443 Positive >1:40/>1:960 21,43% Positive < 1:10
Serum 37 -5,581 Negative n.d. 86,84% Negative < 1:10
Serum 38 -4,913 Negative <1:5 88,36% Negative < 1:10
Serum 39 35,538 Positive >1:40/1:480 6,68% Positive < 1:10
Serum 40 30,291 Positive >1:40/1:960 17,62% Positive < 1:10
Serum 41 -5,200 Negative <1:5 85,33% Negative < 1:10
Serum 42 -3,864 Negative n.d. 86,76% Negative < 1:10
Serum 43 -5,486 Negative n.d. 87,18% Negative < 1:10
Serum 44 -3,959 Negative n.d. 87,91% Negative < 1:10
Serum 45 -5,200 Negative n.d. 86,37% Negative < 1:10
Serum 46 13,691 Positive >1:40/>1:960 3,07% Positive < 1:10
  1. In bold = TBEV-positive horses would result in false positive outcomes in the commercial WNV-ELISA, but clearly negative in the WNV-specific VNT.
  2. n.d. = not done.