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Table 1 Cross-reactivities of TBEV-ELISA and TBEV-VNT results

From: Tick-borne encephalitis virus (TBEV) – findings on cross reactivity and longevity of TBEV antibodies in animal sera

Sample number

Animal

Antibodies classified as (ND50)

TBEV-ELISA

TBEV-VNT (ND50)

Serum 1

Sheep

Louping ill-positive

Positive

Positive (1:20)

Serum 2

Sheep

Louping ill-positive

Negative

Negative (1:5)

Serum 3

Sheep

Louping ill-positive

Positive

Positive (>1:40)

Serum 4

Sheep

Louping ill-positive

Positive

Positive (>1:40)

Serum 5

Sheep

Louping ill-positive

Positive

Positive (>1:40)

Serum 6

Horse

WNV positive (1:320)

Negative

Negative (<1:10)

Serum 7

Horse

WNV positive (1:480)

Negative

Negative (<1:10)

Serum 8

Horse

WNV positive (1:1280)

Borderline

Positive (1:40)

Serum 9

Horse

WNV positive (1:160)

Negative

Negative (<1:10)

Serum 10

Horse

WNV positive (1:240)

Negative

Negative (<1:10)

Serum 11

Horse

WNV positive (1:60)

Borderline

Positive (1:80)

Serum 12

Horse

WNV positive (1:40)

Negative

Negative (<1:10)

Serum 13

Horse

WNV positive (1:80)

Negative

Negative (<1:10)

Serum 14

Horse

WNV positive (1:35)

Negative

Negative (<1:10)

Serum 15

Horse

WNV positive (1:120)

Negative

Negative (<1:10)

Serum 16

Horse

WNV positive (1:60)

Negative

Negative (<1:10)

Serum 17

Horse

WNV positive (1:80)

Negative

Negative (<1:10)

Serum 18

Horse

WNV positive (1:240)

Negative

Negative (<1:10)

Serum 19

Horse

WNV positive (1:20)

Negative

Negative (<1:10)

Serum 20

Horse

WNV positive (1:40)

Negative

Negative (<1:10)

Serum 21

Horse

WNV positive (1:60)

Negative

Negative (<1:10)

Serum 22

Horse

WNV positive (1:1280)

Negative

Negative (<1:10)

Serum 23

Chicken

WNV positive (1:500)

Negative

Negative (<1:10)

Serum 24

Chicken

WNV positive (1:250)

Negative

Negative (<1:10)

Serum 25

Chicken

WNV positive (1:80)

Negative

Negative (<1:10)

Serum 26

Chicken

WNV positive (1:900)

Negative

Negative (<1:10)

Serum 27

Duck

WNV positive (1:80)

Negative

Negative (<1:10)

Serum 28

Duck

WNV positive (1:60)

Negative

Negative (<1:10)

Serum 29

Rabbit

WNV positive (1:120)

Negative

Negative (<1:10)

Serum 30

Rabbit

WNV positive (1:480)

Negative

Negative (<1:10)

Serum 31

Rabbit

WNV positive (1:80)

Negative

Negative (<1:10)

Serum 32

Rabbit

WNV positive (1:60)

Negative

Negative (<1:10)

Serum 33

Rabbit

JEV positive (1:60)

Negative

Negative (<1:10)

Serum 34

Rabbit

JEV positive (1:80)

Negative

Negative (<1:10)

Serum 35

Rabbit

USUV positive (1:1280)

Negative

Negative (<1:10)

Serum 36

Rabbit

USUV positive (1:80)

Negative

Negative (<1:10)

Ascites 1

Mouse

RSSEV-positive

Positive*

Positive (1:75)

Ascites 2

Mouse

POWV-positive

Negative*

Negative (<1:25)

Ascites 3

Mouse

YFV-positive

Negative*

Negative (<1:25)

Ascites 4

Mouse

JEV positive

Negative*

Negative (<1:25)

Ascites 5

Mouse

SLEV positive

Negative*

Negative (<1:25)

  1. *all samples diluted 1:10, 1:50, 1:100, 1:500, 1:1000, only results 1:10 are shown in the table, all samples diluted between 1:50 and 1:1000 were negative.