Illustration to areas of inflammation in the renal tissue of treatment group. A) CaOx crystals deposition (black arrows), tubular injury and inflammatory cells infiltration in the renal tissue, Pizzolatos and HE stains. B) Encircled area shows leukocytic infiltration, fibrin deposition and CaOx crystals retention (black arrow). HE stains. C) Square encloses the area of leukocytic infiltration and OPN expression. Antigens were detected using antibodies to osteopontin, followed by second antibodies coupled to horseradish peroxidase with DAB counterstained with hematoxelyne × 400.