TY - JOUR AU - Mohamaden, Walaa AU - Wang, Heng AU - Guan, Huawei AU - Meng, Xia AU - Li, Jianji PY - 2014 DA - 2014/03/17 TI - Immunohistochemical localization and mRNA quantification of osteopontin and Tamm-Horsfall protein in canine renal tissue after potassium oxalate injection JO - BMC Veterinary Research SP - 70 VL - 10 IS - 1 AB - Urinary macromolecules contribute to promoting or inhibiting crystal retention in renal tissue and stone formation. Osteopontin (OPN) and Tamm-Horsfall protein (THP) are the most important proteins involved in this process. Although these two proteins were discovered a long time ago, their role in setting kidney stone formation has not yet been fully investigated. We conducted a study to explore the role of OPN and THP in canine renal oxalosis. Ten dogs were carefully examined prior to the study. Six dogs were assigned to the treatment group and were injected intravenously with 0.5 M potassium oxalate (KOx). The other four dogs were assigned to a control group and were injected intravenously with 0.9% NaCl three times a day (tid) for 7 consecutive days. Then kidneys were harvested for pathological, immunohistochemical examination and OPN and THP mRNA expression levels were quantified by quantitative real-time PCR. SN - 1746-6148 UR - https://doi.org/10.1186/1746-6148-10-70 DO - 10.1186/1746-6148-10-70 ID - Mohamaden2014 ER -