Skip to main content

Table 1 Sequences of primers and probes used in real-time RT-PCR analysis

From: Substitution of dietary fish oil with plant oils is associated with shortened mid intestinal folds in Atlantic salmon (Salmo salar)

Gene

Gene Sequence 5′ → 3′

GenBank accession no.

Pyloric caeca

Mid intestine

Distal intestine

EF1AB

F-TGCCCCTCCAGGATGTCTAC

BG933853

18.19 ± 0.09

19.74 ± 0.28

18.90 ± 0.15

R-CACGGCCCACAGGTACTG

P-FAM-AAATCGGCGGTATTGG-MGB

CD3ζ

F-AACAGGGATCCAGAGAGTGCTG

BT060238

27.77 ± 0.16

27.74 ± 0.28

27.41 ± 0.20

R-AAGGGACGTGTAAGTGTCGTCA

P-FAM-ACGGCACGCGATAATCGCAGGA-BHQ

COX-2a

F-CAGATCGCTGGAAGGGTGG

AY848944

33.66 ± 0.31

33.32 ± 0.43

32.83 ± 0.35

R-TCATGTTGAAGCGTTTCCTGTAG

    

P-FAM-AGCTAAGGCCCTGGAGCACAGC-BHQ

IgM

F-TGTAAAGAGAGCAGACTGGGACAG

Y12456

25.60 ± 0.55

24.89 ± 0.60

24.63 ± 0.45

R-GAGACGGGTGCTGCAGATATTC

Y12457

P-FAM-TGTTCCACGGCGCATTCAAAGATTT-BHQ

IgT

F-CAGCAGTCTGCTGAAGGTC

GQ907004

29.91 ± 0.29

30.29 ± 0.50

28.00 ± 0.30

R-GGTTCTGTTTTGGAGATCG

GQ907003

P-FAM-CTGCACCACACAGCTGTACTTGACC-BHQ

IL-1β

F-GCTACCACAAAGTGCATTTG

AY617117

34.91 ± 0.42

33.91 ± 0.64

31.98 ± 0.33

R-GAGGTTGGATCCCTTTATGC

P-FAM-CCATTGAGACTAAAGCCAGACCTGTAG-BHQ

MHCII

F-CCACCTGGAGTACACACCCAG

X70165

20.95 ± 0.25

20.34 ± 0.35

20.49 ± 0.18

R-TTCCTCTCAGCCTCAGGCAG

P-FAM-TCCTGCATGGTGGAGCACATCAGC-BHQ

NOD2

F-GCATCCAGTGTGAGCACTTTCAG

EG915470

32.01 ± 0.19

31.55 ± 0.24

31.86 ± 0.23

R-TTCATCTTCAGGAGGTGAGCG

P-FAM-CAAGCTAACTGATGCCTGCACAGAGTGC-BHQ

TGF-β

F-TGGAGCTGAGTGAGGAGCAG

EU082211

34.04 ± 0.29

34.24 ± 0.41

33.46 ± 0.32

R-ACCGCATCTCAGACATGTTG

P-FAM-TGTGGACCTCCTTTGCAAAGTATGC-BHQ

TNF-α

F-GCAGCTTTATGTGCGGCAG

NM_001123589

36.38 ± 0.30

NA

35.47 ± 0.29

R-TTTTGCACCAATGAGTATCTCCAG

NM_001123590

P-FAM-TGGAAGACTGGCAACGATGCAGGA-BHQ

  1. The mean Ct-values ± SEM for the fish oil group are given for each intestinal segment.
  2. NA, Not applied.