TY - JOUR AU - Gabrielyan, Anastasia AU - Knaak, Sven AU - Gelinsky, Michael AU - Arnhold, Stefan AU - Rösen-Wolff, Angela PY - 2014 DA - 2014/03/04 TI - Hypoxia-conditioned media allows species-specific attraction of bone marrow stromal cells without need for recombinant proteins JO - BMC Veterinary Research SP - 56 VL - 10 IS - 1 AB - In vivo tissue regeneration depends on migration of stem cells into injured areas, their differentiation into specific cell types, and their interaction with other cells that are necessary to generate new tissue. Human mesenchymal stem cells, a subset of bone marrow stromal cells (BMSCs), can migrate and differentiate into osteoblasts in bone tissue. This can be facilitated by recombinant growth factors and cytokines. In many animal species, the availability of genomic sequences, recombinant proteins, and/or antibodies is limited so that new approaches are needed to generate resources that facilitate migration of stem cells into tissue defect areas. Here we used bone marrow stromal cells of human, ovine, equine, and canine origin to generate hypoxia-conditioned media (HCM) in order to attract BMSCs of the respective species in migration assays. SN - 1746-6148 UR - https://doi.org/10.1186/1746-6148-10-56 DO - 10.1186/1746-6148-10-56 ID - Gabrielyan2014 ER -