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Table 2 LOD determination of Matrix-Lysis tissue preparation using plate count method (PCM) for enumeration from 5 g of cerebral tissue

From: Quantification of Gram-positive bacteria: adaptation and evaluation of a preparation strategy using high amounts of clinical tissue

CFU1control2

CFU per plate after ML3

Sum of CFU after ML

51 ± 3

21, 9, 11, 5, 11

57

4.5 ± 0.5

2, 0, 1, 1, 0

4

0.5 ± 0.5

1, 0, 0, 0, 0

1

18 ± 1

2, 10, 5, 10, 4

31

0.5 ± 0.5

0, 1, 0, 0, 0

1

0 ± 0

0, 0, 0, 0, 0

0

20 ± 5

4, 5, 7, 4, 1

21

1 ± 0

0, 3, 0, 0, 0

3

0 ± 0

0, 0, 0, 0, 0

0

  1. 1CFU: Colony forming units.
  2. 2PCM was used prior to Matrix-Lysis on TSA + Y for the spiking control.
  3. 3Recovery rates after Matrix-Lysis were determined by subdividing the remaining pellet into five equal parts and plating them individually onto ALOA agar.