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Table 1 Remaining pellet size (% wet weight) after performing Matrix-Lysis protocol with different lysis buffers and tissues

From: Quantification of Gram-positive bacteria: adaptation and evaluation of a preparation strategy using high amounts of clinical tissue

 

8 M urea + 1% SDS (%)

2 M MgCl2 + 1% Lutensol (%)

2 M MgCl2(%)

 

Mayrl et al. (2009) [26]

This study

Mayrl et al. (2009) [26]

This study

Mayrl et al. (2009) [26]

This study

Cerebrum

1.1 ± 0.12

n.e.

2.9 ± 0.05

n.e.

1.4 ± 0.20

n.e.

Spinal cord

1.3 ± 0.11

n.e.

2.5 ± 0.17

n.e.

1.7 ± 0.25

n.e.

Liver

1.8 ± 0.31

n.e.

7.2 ± 0.24

4.3 ± 0.10

8.2 ± 0.21

3.9 ± 0.04

Colon

2.7 ± 0.16

n.e.

9.8 ± 0.03

2.2 ± 0.13

5.2 ± 0.80

n.e.

Kidney

1.0 ± 0.00

n.e.

7.2 ± 0.43

4.1 ± 0.03

3.4 ± 0.25

n.e.

Lung

13.2 ± 0.20

9.8 ± 0.121

23 .0 ± 0.18

7.3 ± 0.171

56.0 ± 0.35

6.6 ± 0.43

Ileum

9.8 ± 0.30

2.6 ± 0.11

47.0 ± 0.09

2.9 ± 0.05

52.0 ± 0.20

3.1 ± 0.05

Caecum

12.1 ± 0.15

3.8 ± 0.10

3.7 ± 0.04

n.e.

12.0 ± 0.24

4.3 ± 0.10

Muscle

1.7 ± 0.08

n.e.

26.7 ± 0.47

11 .0 ± 0.311

5.9 ± 0.02

n.e.

  1. 1lysis efficiency was too low for sufficient solubilization; these pellets could not be used for further downstream analyses.
  2. n.e. = not examined (the protocol of Mayrl et al. (2009) [26] revealed sufficient solubilization).
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BMC Veterinary Research

ISSN: 1746-6148

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