Table 4 Characteristics of gene specific primers used for qPCR

Reference genes

ATP5B

GGACTCAGCCCTTCAGCGCC GCCTGGTCTCCCTGCCTTGC

229

NM_175796.2

PPIA

GGCAAATGCTGGCCCCAACACA AGTACCACGTGCTTGCCATCCA

87

NM_178320.2

RPL12

CACCAGCCGCCTCCACCATG CGACTTCCCCACCGGTGCAC

84

NM_205797.1

Target genes

ATF4

TGGTCTCAGACAACAGCAAG AGCTCATCTGGCATGGTTTC

130

NM_001034342.2

ATM

GTGTTGAGGCACTTTGTGATGC GTTTGATAATGGGCTGGTCTGC

111

NM_001205935.1

BAK1

TACTTCACCAAGATCGCGTC ACGATGGCTACGCTCTTGAT

254

NM_001077918.1

BAX

TCTGACGGCAACTTCAACTG ATGGTCACTGTCTGCCATGT

224

NM_173894.1

BRCA1

TGCCGAGACAAGATCAAGAGG TAATTTCAGTGCAGAGGCTGAGG

149

NM_178573.1

CASP3

CCGAGGCACAGAACTGGACTG TCGCCAGGAAAAGTAACCAGGTG

133

NM_001077840.1

CASP8

TACCAGCGAGGAGGAGATGAAG CATCCAGCTTACATTTGGCAATC

164

NM_001045970.2

CASP9

AAACAGGATGACCCATCAAAGC ATTCAGGACATAGGCCAGATCG

203

NM_001205504.1

DDIT3

AGTCACTGCCTTTCTCCTTC TCTTCCTCCTTGTTTCCAGG

133

NM_001078163.1

DNAJC3

GTACGAAGGTGCTGAATGTG ATCAGGGTCACCATCTACTG

133

NM_174756.3

EDEM1

CCCCTACCCTCGGGTGAATCT GTGGAATCCCCCAGCAGTCG

126

NM_001103092.2

HERPUD1

CCGTGTTTCTCAGTATCCTC TCTTGATTCACAGCCTCCTG

169

NM_001102265.2

HSBP1

CGCGAACAAACGGAAGTATAGG CAGGTCATCAATGCGACTGC

207

NM_001113316.2

HSPA5

CAAGTTGATGTTGGAGGTGG AAGCCTCAGCAGTTTCCTTC

94

NM_001075148.1

HSPA8

AACGTGCTGATCTTTGATTTAGGG TTCTCCACCCAAGTGAGTATCTCC

114

NM_174345.4

MSH2

AACAGAAAGCCCTGGAGTTGG TTATTCTTTGCGATGACCTCAGC

226

NM_001034584.1

PDIA4

AGGTTTGACGTGAGTGGCTA CATCGAAGTTGTCCTTGGTC

175

NM_001045879.2

RPS9

GTGAGGTCTGGAGGGTCAAA GGGCATTACCTTCGAACAGA

108

NM_001101152.2

WARS

AAGCAGACGAGGACTTTGTG TTCGGTTTACCAGCTCCTTG

123

NM_174218.1

XBP1 unspliced

GTTGAGACAGCGGTTGGGAATG CGTAGTCTGAGTGCTGCGGAC

114

NM_001034727.3

XBP1 spliced & unspliced

TGACTGAAGAGGAAGCAGAG CAATGCCATCAGAGTCCATG

129/155

NM_001271737.1

XRCC5

GTTTCAGTGTCTGCTTCACAGAGC TTCTTGATGACTTCCGTCAGAGG

165

NM_001102141.1