A recombinant chimera comprising the R1 and R2 repeat regions of M. hyopneumoniae P97 and the N-terminal region of A. pleuropneumoniaeApxIII elicits immune responses

Table 1 Oligonucleotide primers used for PCR

Primer	Sequence (5' to 3')	Accession #	Gene	Length (bp)
ApxN-F	GGGGATCCGGCTACGATGTAACTAAAAATGGT	L12145	apxIIIA	315
ApxN-F	BamHI restriction sequence italicized
ApxN-R	GGCTCGAGTTATTGTAAGAACTGATCCAGTTGCGG
ApxN-R	XhoI restriction sequence italicized
P97C-F	CGGGATCCAAACTGGATGACAACCTCCAA	U50901	P97	954
P97C-F	BamHI restriction sequence italicized
P97C-R	GGCTCGAGTTAAGGATCTCCGGATTTGCTGTCGTC
P97C-R	XhoI restriction sequence italicized
Ap97-F	GGCCATATGCAAGTTAAAAAAGGCTACGATGTAAC	L12145	apxIIIA	333
Ap97-F	NdeI restriction sequence italicized
Ap97-R	GGCGGATCCATGTTTTTGTTAGAACTGATCCAGTTG
Ap97-R	BamHI restriction sequence italicized
AppCPS-F	ACTATGGCAATCAGTCGATTCAT	AY357726	capsular polysaccharide biosynthesis	500
AppCPS-R	CCTAATCGG AAACGCCATTCTG	AY357726	capsular polysaccharide biosynthesis	500
Mhyop102-F	GTCAAAGTCAAAGTCAGCAAAC	AF012905	P102	137
Mhyop102-R	AGCTGTTCAAATGCTTGTCC

ISSN: 1746-6148