Procedures for bone marrow collection and surgical techniques for segmental defects establishment and stem cell transplantation. A) Puncture procedure of the left iliac crest for obtaining bone marrow aspirate. B) Monoplanar assembly positioned bilateral to external fixator. C) Creation of a 3.5 cm segmental defect in the middle third of the right tibial shaft. D) Cells were injected through the holes in the cortical graft to fill the bone defect. E) Cell suspension injection through the holes in the cortical graft. F) Suture of the incision of the periosteum at the end of the surgical procedure to prevent overflow of cell suspension.