Figure 2From: A SYBR Green I based real time RT-PCR assay for specific detection and quantitation of Peste des petits ruminants virusThe detection limit and standard curve of real time RT-PCR assay based on plasmid copy number. (a) Amplification plot of 10-fold serial diluted plasmid DNA ranging from 5.11 × 107 to 5.11 × 10° copies/μl. (b) Melting curve analysis. (c) Standard curve.Back to article page