Figure 2From: Polymorphisms within the Telomerase Reverse Transcriptase gene (TERT) in four breeds of dogs selected for difference in lifespan and cancer susceptibilityPhylogenetic trees. Unrooted phylogenetic trees showing relationships among (A) the initial set of 20 dogs sequenced from amplicons 1–40, 66–75 as displayed in Figure 1 and (B) a separate set of 20 dogs sequenced using amplicons 41–65. Trees were constructed using MrBayes [16] on multiple sequence alignments that were aligned with clustalx [14] and purged of gaps and ambiguous columns as outlined in Materials and Methods. Posterior probabilities for nodes with greater than 50% likelihood are indicated with branch labels. Terminal leaves are labeled according to dog breed with numbers arbitrarily assigned to individuals within a breed. Specifically, DH1-5: Dachshunds, IW1-5: Irish Wolfhounds, NF1-5: Newfoundlands, and ST1-5: Shih Tzus. There is no overlap between individuals labeled in Figures A and B. In Figure B, A = IW4, IW3, IW2, ST1; B = IW1, ST3; C = PS, ST4; D = NF5, DH4, DH5; E = DH2, DH3; F = ST5, NF1; G = NF4, DH1; H = NF3; I = NF2; J = ST2; K = IW5.Back to article page