Effect induced by FMDV with different chemicals on T lymphocyte subsets of mouse spleen. The proportions of CD3+CD4+ and CD3+CD8+ subsets were assessed by two-color flow cytometry analysis. (a) CD3+CD4+ T cells from immunized mouse spleen. (b) CD3+CD8 +T cells from immunized mouse spleen. One asterisk indicates that p < 0.05 when comparing results from the ISA206, F + A, F + A + P, F + A + R, F + A + P + R, or PHA groups to the PBS group. Two asterisks indicate p < 0.05 when comparing results from the F + A, F + A + P, F + A + R, or F + A + P + R groups to the ISA206 group. All experiments were obtained in triplicate, and data shown are the mean ± standard error. Representatives from three independent experiments are shown.