Enhancement of anti-FMDV IgG antibody by the immunization of FMDV antigens together with R848 and poly(I:C). Mice were bled at different dpv’s. Sera from three mice were pooled together within the same group, and total IgG anti-FMDV antibody titers were detected using ELISA. One asterisk indicates that p < 0.05 when comparing results from the ISA206, F + A, F + A + P, F + A + R, or F + A + P + R groups to the PBS group. Two asterisks indicate p < 0.05 when comparing results from the ISA206, F + A + P, F + A + R, or F + A + P + R groups with the F + A group. Each bar represents the mean antibody titer ± standard error of 12 mice per group.