Quantification of basal TKR and signaling node phosphorylation of cpAC and normal lung tissue. (A) Representative captured fluorescent image of a slide with four phosphorylation array profiles from paired normal and tumor protein lysates of two dogs. Differences between tumor and normal tissue fluorescence intensities for Akt/PKB/Rac at Thr308 are highlighted in the array set from the first dog, whereas differences between tumor and normal tissue fluorescence intensities for p44/42 MAPK are highlighted in the array set from the second dog (white arrows). The black arrow represents one row of the array spotted with three positive (black spots) and two negative (non-colored spots) controls. The entire array has 10 positive controls. (B-D) The protein array measured the phosphorylation status of the selected TKRs that were evaluated using IHC as well as 24 additional TKRs and 11 downstream signaling nodes. The phosphorylation measurement of PDGFR includes pan phosphorylation of both the alpha and beta forms of this receptor. Spot intensities were quantified using ImageQuant™ TL array analysis software. Each bar represents the mean fluorescence intensities obtained and therefore phosphorylation of 12 tumor (black) and 12 normal (grey) normal lung lysates from each dog. Error bars, mean ± SEM. *cpAC vs. normal: P < .05.