Antimicrobial resistance and characterisation of staphylococci isolated from healthy Labrador retrievers in the United Kingdom

Table 1 Details of PCR assays used in this study for nuc , tuf and mec A gene identification

Primer	Sequence (5′-3′)	Amplicon size (bp)	Annealing Temperature (°C)	Control strain	Reference
au-F3	TCGCTTGCTATGATTGTGG	359	57	S. aureus ATCC®25923 (LGC Standards, Teddington, UK)	[77]
au-nucR^*	GCCAATGTTCTACCATAGC	359	57	S. aureus ATCC®25923 (LGC Standards, Teddington, UK)	[77]
pse-F2	TRGGCAGTAGGATTCGTTAA	926	57	S. pseudintermedius (clinical isolate)
pse-R5^*	CTTTTGTGCTYCMTTTTGG	926	57	S. pseudintermedius (clinical isolate)
SSnucF	AATGGCTACAATGATAATCACTAA	526	57	S. schleiferi subspecies coagulans ATCC®49545
SSnucR^*	CATATCTGTCTTTCGGCGCG	526	57	S. schleiferi subspecies coagulans ATCC®49545
tuf-F	GCCAGTTGAGGACGTATTCT	412	55	S. epidermidis ATCC®12228	[105]
tuf-R	CCATTTCAGTACCTTCTGGTAA	412	55	S. epidermidis ATCC®12228	[105]
mecAF	TGGCTATCGTGTCACAATCG	310	55	MRSA (clinical isolate)	[103, 104]
mecAR	CTGGAACTTGTTGAGCAGAG
mA1	TGCTATCCACCCTCAAACAGG	286	57
mA2	AACGTTGTAACCACCCCAAGA

ISSN: 1746-6148