Figure 2

Photomicrographs of TH and AVT immunohistochemical staining in the hypothalamus. A) Photomicrographs of tyrosine hydroxylase neuronal cells in the periventricular area (Pe) of the hypothalamus, based on figure 25 of the stereotaxic atlas of the chick brain (Puelles et al. [48]), B) Photomicrographs of vasotocin neuronal cells in the medial pre-optic area (MPA), based on figure 14 of the stereotaxic atlas of the chick brain (Puelles et al. [48]) and C) Photomicrographs of vasotocin neuronal cells in the parvocellular part of the paraventricular nucleus (PaPC), based on figure 16 of the stereotaxic atlas of the chick brain (Puelles et al. [48]). Left to Right: CL NO = control genetic line raised without a hen, CL HEN = control genetic line raised with a hen, LML NO = low mortality genetic line raised without a hen, LML HEN = low mortality genetic line raised with a hen. All images were taken at 10x magnification. Abbreviations: 3v = third ventricle, ac = anterior commissure, ir = infundibular recess, MPO = medial pre-optic nucleus, MPA = Medial pre-optic area, PaPC = parvocellular area of the paraventricular nucleus, Pe = periventricular area.