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Figure 6 | BMC Veterinary Research

Figure 6

From: Biologic activity of the novel orally bioavailable selective inhibitor of nuclear export (SINE) KPT-335 against canine melanoma cell lines

Figure 6

Confocal microscopy to detect p21 and p53 cellular localization following KPT-335 treatment of melanoma cell lines. The canine melanoma cell lines Mel 23 and Mel 69 were treated with DMSO or 1 μM KPT-335 for 24 hours. Cells were fixed and stained with ant-p21 (A) or anti-p53 (B) and then a secondary FITC conjugated antibody. Cells were then counterstained with DAPI to identify the nucleus. Images were obtained using an Olympus FV1000 Spectral confocal microscope and overlayed to assess protein localization.

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BMC Veterinary Research

ISSN: 1746-6148

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