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Table 5 FTIR and MALDI-TOF MS identification results of blind study

From: Novel physico-chemical diagnostic tools for high throughput identification of bovine mastitis associated gram-positive, catalase-negative cocci

No of isolates

16S rRNA sequencing

Identification accuracy

Comment

FTIR ( n)

MALDI-TOF MS( n) Log(score) value ≥2.3

1

A. viridans

1/1

1/0

Match (0%)

3

E. faecalis

3/3

3/3

Match (100%)

2

L. garvieae

2/2

2/1

Match (50%)

1

L. lactis

1/1

1/1

Match (100%)

3

S. dysgalactiae

3/3

3/3

Match (100%)

11

S. uberis

11/11

11/11

Match (100%)

21

 

21/21

21/19

Match (90.5%)

  1. Blind validation study to assess the suitability of FTIR and MALDI-TOF MS for routine diagnosis of mastitis associated Streptococcus spp. and related species. 21 isolates, provided by TgD Styria and Upper Austria, were used for this blind test.