Novel physico-chemical diagnostic tools for high throughput identification of bovine mastitis associated gram-positive, catalase-negative cocci

Table 2 Oligonucleotide primers used for species-specific PCR

Species	Oligonucleotide sequences (5′-3′)	Target gene	PCR-product (bp)	Reference
E. faecalis	ATCAAGTACAGTTAGTCT	genes encoding D-Ala:D-Ala ligases	941	[25]
E. faecalis	ACGATTCAAAGCTAACTG		941
E. faecium	GCAAGGCTTCTTAGAGA		550
E. faecium	CATCGTGTAAGCTAACTTC		550
	GTTGAGCCACTGCCTTTTAC	16 S rRNA	252	[26]
L. garvieae	AATGGGGGCAACCCTGA
L. lactis	GTTGTATTAGCTAGTTGGTGAGGTAAA		387
S. agalactiae	AACAGCCTCGTATTTAAAATGATAGATTAAC	23S rRNA	866	[6]
	TCCTACCATGACACTAATGTGTC
S. bovis	CCCGGCATGTAATGCATGTC		169
	TACAACCCCGATGTGTAAACACA
S. canis	AAGGAAATGGAACACGTTAGGG		924
	CTCCTACCATTACCTCTTAAGGTA
S. dysgalactiae	AAGGAAATGGAACACGTTAGGG		1508
	TCCTACCATGACACTAATGTGTC
S. parauberis	GAA ACT CTT CTA GCA GAC GTG GAA TC	Superoxide Dismutase A (sod A)	254	[27]
S. parauberis	CCT TCT TTA TTT ACA ACT AGC CAT GCC		254
S. uberis	TGG CGT TAT TAT CTG ATG TGT CAT CA		321
S. uberis	CCA AAA TAG GCT GTT TAC CTT CAG AA		321
S. pyogenes	AAAGACCGCCTTAACCACCT	S. pyogenes-specific gene Spy1258	407	[28]
S. pyogenes	TGGCAAGGTAAACTTCTAAAGCA	S. pyogenes-specific gene Spy1258	407	[28]

ISSN: 1746-6148