Table 3 Primers used in this study
Primer use and primer | Sequence (5′-3′) | Reference |
|---|---|---|
Detection tet (A) | ||
Tet(A)-F | GCTACATCCTGCTTGCCTTC | [27] |
Tet(A)-R | CATAGATCGCCGTGAAGAGG | [27] |
Detection tet (B) | ||
Tet(B)-F | TTGGTTAGGGGCAAGTTTTG | [27] |
Tet(B)-R | GTAATGGGCCAATAACACCG | [27] |
Detection tet (M) | ||
Tet(M)-1 (266) | GTTAAATAGTGTTCTTGGAG | [16] |
Tet(M)-2 (267) | CTAAGATATGGCTCTAACAA | [16] |
Detection Tn 916 -like ( xis - Tn ) | ||
Tn916-1 (327) | GCCATGACCTATCTTATA | [20] |
Tn916-2 (328) | CTAGATTGCGTCCAA | [20] |
Detection Tn 5397 -like ( tndX ) | ||
Tn5397-tndX-1 (864) | ATGATGGGTTGGACAAAGA | [20] |
Tn5397-tndX-2 (865) | CTTTGCTCGATAGGCTCTA | [20] |
Detection Tn 5801 -like ( int ) | ||
intcw459-1 (1811) | CCGATATTGAGCCTATTGATGTG | [22] |
intcw459-2 (1812) | GTCCATACGTTCCTAAAGTCGTC | [22] |
Amplification and sequencing tet (M) | ||
TetM-upstream (526) | TTGAATGGAGGAAAATCAC | [20] |
TetM-up (323) | CTGGCAAACAGGTTC | [20] |
TetM sequence-1 (525) | TACTTTCCCTAAGAAAGAAAGT | [20] |
TetM sequence-3 (540) | GCAGAAATCAGTAGAATTGC | [20] |
TetM sequence-6 (709) | TCGAGGTCCGTCTGAAC | [22] |
Reverse TetM-2 (307) | TTGTTAGAGCCATATCTTAG | [20] |
TetM sequence-9 (1756) | AACAGTAAAATGTATAGAGGTG | [22] |
F2R (1837) | GTGTCTTATACCATGGAAGGA | [22] |
TetM-down (324) | TAGCTCATGTTGATGC | [22] |
Tet(M)-1 (266) | GTTAAATAGTGTTCTTGGAG | [16] |