Table 1 Sequence of primers used for Q-PCR analysis
Gene | Sense | Antisense | Accession number |
|---|---|---|---|
Housekeeping | |||
GAPDH | 5’ CCTTCATTGACCTTCACTACATGGTCTA | 5’ TGGAAGATGGTGATGGCCTTTCCATTG | NM 001034034.2 |
βACTIN | 5’ CGCACCACTGGCATTGTCAT | 5’ TCCAAGGCGACGTAGCAGAG | K00622.1 |
Hepatogenic | |||
AFP | 5’ CTTGTTGCTTACACAAAGAAGG | 5’ ATGGAAGATGAACTTGTCATCG | NM 001034262.2 |
ALB | 5’ TTTTCTCAGTATCTCCAGCAGT | 5’ AGTAGGGATGTCTTCTAGCAAT | NM 180992.2 |
α1AT | 5’ GCTGGGGTTCTCCAAGGAC | 5’ GTTTGCTCATTCACGTGGAAGTC | NM 173882.2 |
CNX32 | 5’ ATGAACTGGACAGGTTTGTAC | 5’ ATGTGTTGCTGGTGAGCCA | NM 174069.2 |
CYP3A4 | 5’ ATCATTGCTGTCTCCAACCTTCAC | 5’ TGCTTCCCGCCTCAGATTTCTC | NM 001099367.1 |
TAT | 5’ TTTGCTATGGAGCTTTGGCTGC | 5’ AATGGTAGTGCAGCTCGCAGAA | NM 001034590.1 |
Neurogenic | |||
NESTIN | 5’ CTGGAGCAGGAGAAACAAGG | 5’ GAAAGGTTGGCACAGGTGTT | NM 001206591.1 |
MAP2 | 5’ CCACCTGAGATTAAGGATCA | 5’ GGCTTACTTTGCTTCTCTGA | NM 001205807.1 |
NGF | 5’ TCAACAGGACTCACAGGAGCAA | 5’ ACCTCTCCCAGCACCATCAC | NM 001099362.1 |
TRKA | 5’ CTGGGTGAGGGTGCCTTT | 5’ CGCTCTCAGACACCTCCTTCAG | XM 002685965.3 |
PrPC | 5’ CCAGAGACACAAATCCAACTTGAG | 5’ AACCAGGATCCAACTGCCTATG | NM 001271626.1 |
Pluripotency | |||
NANOG | 5’ GTGTTTGGTGAACTCTCCTG | 5’ GGGAATTGAAATACTTGACAG | NM 001025344.1 |