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Figure 5 | BMC Veterinary Research

Figure 5

From: Hepatogenic and neurogenic differentiation of bone marrow mesenchymal stem cells from abattoir-derived bovine fetuses

Figure 5

Neural gene expression profile in bMSC under differentiating conditions using neurogenic protocol 2. (A) Culture of fetal bMSC under neurogenic conditions induced formation of polygonal cell morphology and cell aggregates at Day 28 of culture. (B) At 24 h of culture, differentiated bMSC expressed both NESTIN and MAP2 proteins; whereas, at 144 h of culture also expressed TRKA and PrPC proteins. (C) MAP2 protein was immunodetected in cell cytoplasm associated to the cytoskeleton. Levels of MAP2 mRNA were up-regulated in differentiated (DIF) bMSC at 144 h of culture. In addition, these cells expressed higher (P < 0.05) levels of TRKA mRNA at 96 and 144 h of culture compared to controls. No differences in mRNA levels were detected for NESTIN, NGF and PrPC between differentiated and control bMSC cultures. NANOG mRNA levels were lower (P < 0.05) in differentiated compared to control bMSC from 96 h to 144 h of culture. (*, a,b) Superscripts represent significant (P < 0.05) differences between treatments and sampling days, respectively. Bar scale: 500 μm.

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