Table 1 Clinical sensitivities of qPCR assays for the detection of the WSSV and the PstDV1
From: Development of duplex real-time PCR for the detection of WSSV and PstDV1 in cultivated shrimp
Sample* | Singleplex sensitivity (%) | Duplex sensitivity (%) | Coinfections (Duplex qPCR) | |||
|---|---|---|---|---|---|---|
TaqMan | QuantiTect | TaqMan | QuantiTect | TaqMan | QuantiTect | |
Cycle threshold ( C q ) | ||||||
WSSV positive (n =28) | 26 (92.85) | 24 (85.71) | 24 (85.71) | 23 (82.14) | 1 | 1 |
PstDV1 positive (n = 5) | 2 (40) | 2 (40) | 2 (40) | 2 (40) | ||
Negative (n = 10) | 0 | 0 | 0 | 0 | ||
Relative threshold (CRT) | ||||||
WSSV positive (n =28) | 24 (85.71) | 24 (85.71) | 28 (100) | 28 (100) | 3 | 3 |
PstDV1 positive (n = 5) | 2 (40) | 5 (100) | 5 (100) | 5 (100) | ||
Negative (n = 10) | 0 | 0 | 0 | 13** | ||