Figure 2

Hoechst 33258 staining of back fat sections (100× magnification). Pig back fat cryosections were fixed in 4% neutral formalin and stained with Hoechst 33258 (10 μg/mL). The number of apoptotic cells (i.e., those showing chromatin condensation and fluorescence enhancement) was assessed by observation with an inverted fluorescence microscope. There were more apoptotic cells present in the 2% CLA-fed pigs than in pigs in the other two groups.