Total IgA, IgG and IgM concentrations in porcine oral fluid samples as determined via Western Blot analysis. Oral fluid samples were collected from one pig (V3) at 14 days post vaccination. The samples were reduced, subjected to SDS-PAGE and transferred to PVDF membranes via Western Blotting. Total IgA, IgG or IgM antibodies were detected using porcine IgA-, IgG- and IgM-specific antibodies. Specific bands of 60 kDa (IgA), 53 kDa (IgG) and 85 kDa (IgM) were detected. ImageJ software was used to quantify the relative amounts of protein and results were plotted relative to antibody concentrations found in unstimulated saliva.