Figure 2From: Detection of total and PRRSV-specific antibodies in oral fluids collected with different rope types from PRRSV-vaccinated and experimentally infected pigsTotal IgA, IgG and IgM concentrations in porcine oral fluid samples as determined via Western Blot analysis. Oral fluid samples were collected from one pig (V3) at 14Â days post vaccination. The samples were reduced, subjected to SDS-PAGE and transferred to PVDF membranes via Western Blotting. Total IgA, IgG or IgM antibodies were detected using porcine IgA-, IgG- and IgM-specific antibodies. Specific bands of 60Â kDa (IgA), 53Â kDa (IgG) and 85Â kDa (IgM) were detected. ImageJ software was used to quantify the relative amounts of protein and results were plotted relative to antibody concentrations found in unstimulated saliva.Back to article page