Electrophoretic analysis of expressed HEV rΔ111ORF2 protein. SDS-PAGE (panel A) and Western blotting (panel B) of crude protein extracts from mock infected Sf9 cells (lane 1) and cells infected with BacHEVΔ111ORF2 (lane 2). After electrophoresis, gels were stained with Coomassie Blue for direct protein visualization, or blotted onto NC paper for protein Immunostaining using an anti-HEV swine immune serum (see Methods). The 55 kDa HEV rΔ111ORF2 protein band is indicated by an arrow. Panel C shows the rΔ111ORF2 protein separated by PAGE and stained with Coomassie Blue, after purification using a Macro-Prep Hydroxyapatite column. MW: molecular weight markers.