Immunized groups | 7 dpi | 14 dpi | 28 dpi | 42 dpi |
---|
pVAX1-EU-ORF3 | <2 | 4.6 ± 0.22 | 7.3 ± 0.68 | 6.8 ± 0.23 |
pVAX1-EU-ORF5 | <2 | 6.4 ± 0.32 | 10.9 ± 0.97 | 16.3 ± 1.45 |
pVAX1-EU-ORF3-ORF5 | <2 | 8.5 ± 0.65 | 14.8 ± 1.28 | 21.1 ± 2.03 |
pVAX1 | <2 | <2 | <2 | <2 |
PBS | <2 | <2 | <2 | <2 |
- Virus-neutralizing (VN) antibody titers in mice after vaccination for different groups. Serum samples (n = 8) were collected at various time-points. PRRSV-specific neutralizing antibodies were detected by a virus neutralizing assay with two-fold serial dilutions. The VN titers were expressed as the reciprocal of the highest serum dilution in which no CPE was observed.