Detection of T cell subgroups of spleens in mice. Splenocytes of mice (n = 8) were harvested and stained as described in Methods section. FACS was used to analyze the percentage of CD3+CD4+ or CD3+CD8+ T cells. Each bar represents the group mean for the percentage of T cell subgroups. * Indicates a significant difference (P < 0.05) between the groups. Data are shown as the mean ± S.D.