Table 1 Classification of the causes for requesting manual leukocyte differentials
From: Rate of manual leukocyte differentials in dog, cat and horse blood samples using ADVIA 120 cytograms
Cause for manual differential | Characteristic findings in the ADVIA 120 cytograms |
|---|---|
1) Suspicion of left shift | - Indistinct separation of polymorphonuclear and mononuclear cells (“worm with swollen neck”) |
- Shortened population of polymorphonuclear cells (“short worm”) in the ADVIA 120 baso cytogram | |
2) Inappropriate separation of cell populations | - Indistinct circumscription or missing separation of the cell populations in the ADVIA 120 peroxidase cytogram |
- Evidence of myeloperoxidase-deficient neutrophils | |
- Cells spreading from the mononuclear or polymorphonuclear gate into the lyse-resistant cell area (baso cytogram) | |
- Discrepancy between the peroxidase and baso cytogram (Figure 3C) | |
3) Suspicion of atypical lymphocytes | - Increased number of cells in the large unstained cell (LUC) gate |
4) Suspicion of abnormal cells (e.g. blasts) | - Increased number of cells in the LUC gate |
- Cells spreading from the mononuclear or polymorphonuclear gate into the lyse-resistant cell area (baso cytogram) | |
- Evidence of a “blast nose” in the baso cytogram |