Sow response to Mycoplasma hyopneumoniae vaccination. M. hyopneumoniae specific AMI (panel A) and CMI (panel B) were measured in sow blood and colostrum. Black bars represent vaccinated sows; gray bars represent nonvaccinated sows; white bars represent sows pre-vaccination; n = 10/group. Error bars are SEM; different subscripts represent significance at p < 0.05. A. M. hyopneumoniae specific antibody S:P ratios were measured in serum and colostrum of vaccinated and nonvaccinated sows using the Idexx HerdCheckTM ELISA. B. M. hyopneumoniae specific CMI was measured in sow colostrum via lymphoproliferation. Antigen specific lymphoproliferation was determined by subtracting unstimulated (media only) lymphoproliferation from M. hyopneumoniae specific lymphoproliferation.