Figure 2

Effect of FBS and HS on cell growth, doubling time, protein content and passage number. EBF were passaged every week and seeded in a density of 2 × 10⁴ cells/cm². EBF cultured in 10% FBS keep proliferating over a longer period than in 10% HS (A). EBF were seeded in 6-well-plates at a density of 2 × 10⁴ cells/cm². Effect of HS and FBS on growth curve (B), population doubling takes longer in HS than in FBS (calculated between day 1 and day 3) (C) and effect of HS and FBS on total protein amount over 8 days (D). Data are presented as means ± SEM. (*p < 0.05, **p < 0.01, n = 10).