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Figure 3 | BMC Veterinary Research

Figure 3

From: High yield production of pigeon circovirus capsid protein in the E. coliby evaluating the key parameters needed for protein expression

Figure 3

Growth kinetics and production profiles of recombinant PiCV capsid protein (Cap) in different E. coli strains. (A) The growth and protein production profiles of the three recombinant E. coli strains expressing either the GST-Cap or GST-Capopt protein. Two strains, BL21 (DE3) and BL21 (DE3)-pLysS expressing GST-Capopt, and one GST-Cap expressing strain BL21 (DE3)-CodonPlus-RIPL were used in this study post-induction. (B) Growth and protein production profiles of four recombinant E. coli strains expressing Trx-His-Cap or Trx-His-Capopt protein. Two E. coli strains, BL21 (DE3) and BL21 (DE3)-pLysS were used to express Trx-His-Capopt, and other two recombinant E. coli strains, BL21 (DE3)-CodonPlus-RIPL and BL21 (DE3)-pLysS were used to express Trx-His-Cap. Similar tests to those described above were used to detect the time course of protein expression after IPTG induction.

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BMC Veterinary Research

ISSN: 1746-6148

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