Reference image 2DE map of differentially expressed spots with marked selected spots. Representative 2-DE map of canine serum obtained by performing the first dimension (IEF) on IPG strips pH 3–10 and the second dimension on 4–12% gradient SDS-PAGE gels. The protein spots were visualized by Coomassie blue staining. The indicated spots were excised from gel and identified by MS/MS. Mr standard values are indicated on the left.