BMC Veterinary Research

Table 1 Real-time PCR reaction conditions

From: Detection of clonal antigen receptor gene rearrangement in dogs with lymphoma by real-time polymerase chain reaction and melting curve analysis

Product	Primer		Amplification conditions
	Name*	Amount per reaction (nM)	Annealing (°C)	Number of cycles
Cμ	Sigmf1	120	60	40
Cμ	Srμ3	120	60	40
IgH major	CB1	120	60	50
IgH major	CB2	120	60	50
IgH minor	CB1	120	60	40
IgH minor	CB3	360	60	40
TCRγ	TCRγ1	60	60	40
TCRγ	TCRγ2	60	60	40
	TCRγ3	360

*Primer names and sequences according to Burnett et al. [6].

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ISSN: 1746-6148

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