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Table 1 Real-time PCR reaction conditions

From: Detection of clonal antigen receptor gene rearrangement in dogs with lymphoma by real-time polymerase chain reaction and melting curve analysis

Product Primer Amplification conditions
  Name* Amount per reaction (nM) Annealing (°C) Number of cycles
Sigmf1 120 60 40
Srμ3 120
IgH major CB1 120 60 50
CB2 120
IgH minor CB1 120 60 40
CB3 360
TCRγ TCRγ1 60 60 40
TCRγ2 60
  TCRγ3 360   
  1. *Primer names and sequences according to Burnett et al. [6].