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Figure 5 | BMC Veterinary Research

Figure 5

From: Detection of clonal antigen receptor gene rearrangement in dogs with lymphoma by real-time polymerase chain reaction and melting curve analysis

Figure 5

Comparison of detection limits between conventional and real-time PCR. Serial dilutions of DNA samples of 18 dogs with B cell lymphoma (A) and 6 dogs with T cell lymphoma (B) were prepared with regular lymphoid DNA (50%, 25%, 12.5%, 6.25%, 3.13%, 1.56%, 0.78%) and amplified with IgH major and TCRγ primer sets by both PCR methods. The number of positive results obtained with PAGE (black squares) and MCA (white squares) is indicated for each concentration.

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