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Figure 5 | BMC Veterinary Research

Figure 5

From: Detection of clonal antigen receptor gene rearrangement in dogs with lymphoma by real-time polymerase chain reaction and melting curve analysis

Figure 5

Comparison of detection limits between conventional and real-time PCR. Serial dilutions of DNA samples of 18 dogs with B cell lymphoma (A) and 6 dogs with T cell lymphoma (B) were prepared with regular lymphoid DNA (50%, 25%, 12.5%, 6.25%, 3.13%, 1.56%, 0.78%) and amplified with IgH major and TCR╬│ primer sets by both PCR methods. The number of positive results obtained with PAGE (black squares) and MCA (white squares) is indicated for each concentration.

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