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Figure 3 | BMC Veterinary Research

Figure 3

From: Detection of clonal antigen receptor gene rearrangement in dogs with lymphoma by real-time polymerase chain reaction and melting curve analysis

Figure 3

Evaluation of 3 patients with B cell lymphoma by MCA and PAGE. Samples of 3 dogs with B cell lymphoma were amplified with Cμ (A) and IgH major (B) primer sets by real-time and conventional PCR. Amplification with Cμ primers revealed comparable peak heights on MCA and distinct bands on PAGE for all dogs. Amplification with IgH major primers showed positive results for all 3 dogs on MCA, based on the cut-off of 1.5:1 of lymphoma to regular lymphoid DNA although peak heights varied significantly. Two of the dogs revealing lower peak heights on MCA (dogs 2,3) failed to show identifiable distinct bands on PAGE. All reactions were carried out with a total of 100 ng of DNA.

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BMC Veterinary Research

ISSN: 1746-6148

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