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Figure 2 | BMC Veterinary Research

Figure 2

From: Detection of clonal antigen receptor gene rearrangement in dogs with lymphoma by real-time polymerase chain reaction and melting curve analysis

Figure 2

Optimization of real-time PCR and MCA for the detection of B and T cell clonal rearrangement. Samples of 3 dogs with B cell lymphoma (A, grey lines) and T cell lymphoma (B, grey lines) were amplified in parallel with regular lymphoid DNA from 3 dogs (black lines) for 30, 40 and 50 cycles. A total of 50 cycles for IgH major and of 40 cycles for TCRγ were found to be optimal for evaluation of clonal rearrangement, based on the peak height of clonal samples and the –d(F1)/dT ratio between lymphoma and non-neoplastic DNA. Peak height was measured for all samples from zero to the highest point of the melting curve.

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BMC Veterinary Research

ISSN: 1746-6148

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