RNAse protection assay (RPA) for various bcl-2 homologue mRNAs from spleens of individual BALB/c mice. A representative RPA of a control mouse 28 days post sham inoculation is shown on the left. RPAs for representative BALB/c mice that were inoculated with a single (sPCV2) or multiple (mPCV2) doses of PCV2 and were euthanized at 14, 28 or 42 days PI, are shown on the right. Transcripts of apoptotic inhibitors bcl-2, bcl-w and bcl-X and apoptotic promoters bax, bak and bad are increased in sPCV and mPCV mice when compared to control mice. In addition, there was also upregulation of transcripts for bfl-1 in sPCV and mPCV mice. Quantitation of mRNA is identical for all samples.