RNAse protection assay (RPA) for various caspase mRNAs from spleens of individual BALB/c mice. The probe and a representative RPA of a control mouse 28 days post sham inoculation are shown on the left. RPAs for representative BALB/c mice that were inoculated with a single (sPCV2) or multiple (mPCV2) doses of PCV2 and were euthanized at 14, 28 or 42 days PI, are shown on the right. Transcripts for caspases 1, 2, 3, 6, 7, 8, 11 and 12 are increased in sPCV and mPCV mice when compared to control mice. Upregulation of caspases 1, 2, 3, 6 and 8 are strongest and bands for caspase 11 and 12 are only faint. Quantitation of mRNA is identical for all samples.