Figure 1
From: Porcine Circovirus type 2 (PCV2) causes apoptosis in experimentally inoculated BALB/c mice
Spleen from a PCV2-inoculated sPCV mouse 28 days PI. Large numbers of apoptotic cells (arrows) in germinal centers. H & E staining, Bar = 40 μm. Same germinal center in spleen as in figure 1. Apoptotic cells (arrows) stain dark brown with TUNEL. In-situ hybridization, hematoxylin counterstain, Bar = 20 μm. Same germinal center in spleen as in figure 1. The majority of apoptotic cells (arrows) stain dark blue for PCV2 nucleic acid. In-situ hybridization, hematoxylin counterstain, Bar = 60 μm