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Table 4 Relative mRNA expression levels of PrP gene (arbitrary units)

From: Selection of ovine housekeeping genes for normalisation by real-time RT-PCR; analysis of PrPgene expression and genetic susceptibility to scrapie

Risk group

 

Cerebrum

Cerebellum

Obex

Spleen

Ileum

Mesenteric lymph node

Nervous tissues

Lymphoid tissues

 

n a

nPrP b (SDc)

nPrP (SD)

nPrP (SD)

nPrP (SD)

nPrP (SD)

nPrP (SD)

nPrP (SD)

nPrP (SD)

1

3

15.829 (4.279)

21.202 (3.052)

29.892 (12.223)

38.851 (16.115)

42.048 (8.800)

31.424 (3.496)

22.308 (9.057)

37.442 (10.470)

2

5

27.132 (4.830)

26.632 (3.495)

37.897 (6.930)

24.504 (16.953)

33.659 (13.712)

31.940 (4.088)

30.554 (7.267)

30.035 (12.551)

3

7

21.025 (5.869)

33.088 (6.515)

42.435 (7.598)

33.468 (18.426)

36.318 (13.434)

36.971 (6.320)

32.183 (11.001)

35.586 (13.054)

5

7

20.645 (5.092)

29.584 (4.439)

37.283 (8.811)

26.628 (10.323)

33.897 (9.467)

32.051 (5.895)

29.171 (9.247)

30.859 (8.903)

Total

22

21.584 (5.991)

28.885 (6.055)

38.054 (8.841)

29.989 (15.268)

35.725 (11.328)

33.506 (5.621)

29.508 (9.736)

33.073 (11.517)

  1. Risk groups are listed from the most resistant one on the top to the most susceptible at the bottom according to the NSP classification [7]. Type 1, included three ARR/ARR animals; Type 2, included five ARR/ARQ animals; Type 3, included one ARH/ARH, one ARQ/ARH and five ARQ/ARQ animals; Type 5, included one ARH/VRQ, five ARQ/VRQ and one VRQ/VRQ animal.
  2. a Number of animals
  3. b Normalised PrP mRNA level
  4. c Standard deviation