Selection of ovine housekeeping genes for normalisation by real-time RT-PCR; analysis of PrPgene expression and genetic susceptibility to scrapie

Table 3 HK genes stability series for each tissue

Cerebrum	Cerebellum	Obex	Spleen	Mesenteric lymph node	Ileum
*GAPDH-SDHA*	*G6PDH-ACTB*	*GAPDH-YWHAZ*	*GAPDH-SDHA*	*SDHA-RPL19*	*GAPDH-G6PDH*
*ACTB*	*YWHAZ*	*G6PDH*	*ACTB*	*GAPDH*	*SDHA*
G6PDH	RPL19	SDHA	YWHAZ	ACTB	*YWHAZ*
RPL19	GAPDH	ACTB	RPL19	G6PDH	RPL19
YWHAZ	SDHA	RPL19	G6PDH	YWHAZ	ACTB

The stability series are shown from the most stable gene at the top to the least stable gene at the bottom ranked to their expression stability estimated using geNorm. The first two genes in each series cannot be ranked because of the required use of gene ratios for gene stability measurements. The HK genes required for a reliable normalisation of the target gene expression in each tissue are shown in bold.

ISSN: 1746-6148