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Table 2 Expression stability values (M) of the six candidate HK genes

From: Selection of ovine housekeeping genes for normalisation by real-time RT-PCR; analysis of PrPgene expression and genetic susceptibility to scrapie

Tissue

ACTB

YWHAZ

RPL19

SDHA

GAPDH

G6PDH

Cerebrum

0.602

0.757

0.634

0.540

0.510

0.660

Cerebellum

0.477

0.480

0.452

0.518

0.473

0.457

Obex

0.534

0.493

0.593

0.541

0.451

0.479

Spleen

0.436

0.413

0.550

0.427

0.448

0.569

Mesenteric lymph node

0.556

0.691

0.542

0.525

0.502

0.624

Ileum

0.757

0.652

0.696

0.610

0.517

0.511

Mean

0.560

0.581

0.578

0.527

0.484

0.550

SD a

0.113

0.137

0.084

0.059

0.030

0.081

CV (%)b

20.133

23.669

14.543

11.192

6.268

14.771

  1. M values in this table were calculated for the six HK genes previous to the stepwise exclusion of the less stable HK gene.
  2. a Standard deviation
  3. b Coefficient of variation