Table 1 PCR reaction mix
From: Milk miRNA expression in buffaloes as a potential biomarker for mastitis
Component | 96-well plate (0.1 ml standard) | Final Concentration |
|---|---|---|
Taqman® Fast Advanced Master Mix (2x) | 10.0 | 1x |
Taqman® Assay Primer/Probe (20x) | 1.0 | 1x |
cDNA template | 2.0 | 0.001–100 ng/well |
Nuclease free water | 7.0 | – |
Total Volume per reaction | 10.0 | 20.0 |