BMC Veterinary Research

Table 2 PCR cycling conditions

From: Comparative biotoxicity study for identifying better alternative insecticide especially green nano-emulsion which used as mosquitocides

Steps	Temperature	Time
Initial denaturation	95 ^oC	10 min
40 cycles
Denaturation	95 ^oC	30 s
Annealing	60 ^oC	30 s
Extension	72 ^oC	45 s
Final extension	72 ^oC	10 min

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ISSN: 1746-6148

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