Fig. 6

Photomicrographs of sections in the liver tissues of different groups of the African-Egyptian toad treated with different nanomaterials: (a) A section in the control group, showing normal histological structure of hepatocytes cord (long arrow), melanomacrophages (short arrow) and central vein (CV). (b) A section in the control group showing normal histological structure of portal area (arrow). (c) A section in the group treated with group D showing mild vacuolar degeneration of hepatocytes (short arrow) and activation of melano-macrophages (long arrow). (d) A section in the group treated with group D, showing focal area of mononuclear inflammatory cells infiltration(long arrow) with hepatocellular vacuolar degeneration (short arrow). (e) A section in the group treated with group D, showing central vein congestion (arrow). (f) A section in the group treated with group D, showing portal area fibrosis and few mononuclear inflammatory cells infiltration (arrow). (g) A section in the group treated with group D, showing massive vacuolation and necrosis of hepatocytes (arrow). (h) A section in the group treated with group E, showing vacuolation (long arrow) and necrosis (short arrow) of hepatocytes, portal fibrosis and hyperplasia of bile ducts (white arrow). (i) A section in the group treated with group E showing multifocal areas of mononuclear inflammatory cells infiltration (arrows). (j) A section in the group treated with group E, showing infiltration of portal areas with mononuclear inflammatory cells infiltration (long arrow) with activation of melano-macrophages (short arrow) (H &E X 400)