Fig. 2
From: An unexpected case of a dog from Poland co-infected with Dirofilaria repens and Dirofilaria Immitis
Melt curve analysis of products amplified using gene-specific primers for D. immitis: (A) reveals a distinct peak with a specific melting temperature (Tm) corresponding to the amplicon obtained from the tested sample; (B) demonstrates primer specificity assessed with genomic DNA isolated from adult D. immitis (Adult Di) and D. repens (Adult Dr) worms, blood from dogs infected with D. immitis (Mf Di), and D. repens (Mf Dr). NTC implies “no target control”