Evaluation of the effect of therapeutic durations on small ruminant bacterial pneumonia

BMC Veterinary Research

Table 1 Primer pairs, amplification capacity and annealing T^o of targeted virulence-associated genes of M. hemolytica and P. multocida used in the study

Gene target	Primer sequence (5′----3′)	Size (bp)	Annealing T^o (°C)	Reference
PHSSA	Forward 5′- TTCACATCTTCATCCTC-3’	325	48	Kumar et al. (2015) [15]
PHSSA	Revers 5′- TTTTCATCCTCTTCGTC-3’	325	48
Rpt2	Forward 5′- GTTTGTAAGATATCCCATTT-3’	1022	48
Rpt2	Revers 5′- CGTTTTCCACTTGCGTGA-3’	1022	48
KMT1T7	Forward 5′-ATCCGCTATTTACCCAGTGG-3’	560	55	Shivachandra et al. (2006) [16]
KMT1SP6	Revers 5′- GCTGTAAACGAACTCGCCAC-3’	560	55	Shivachandra et al. (2006) [16]

ISSN: 1746-6148